High frequency plant regeneration from embryogenic callus of a popular indica rice (Oryza sativa L.)
نویسندگان
چکیده
منابع مشابه
Efficient in vitro plant regeneration via leaf base segments of indica rice (Oryza sativa L.).
A reliable and reproducible protocol has been developed for high frequency plant regeneration from 4-5 mm long leaf base segments of 4 days old in vitro germinated seedlings of indica rice (Oryza sativa) cultivar Rasi. The effect of age of seedlings, position of leaf base segments and optimum concentration of 2,4-D on callus induction frequency was investigated with a future aim to use leaf bas...
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Callus induction and regeneration in vitro of three rice varieties (Oryza sativa L. ssp indica), Bg 94-1, Moraberekon and Bg 300 were examined. Phenotypic variations of regenerated and parent plants were compared and the method of plant regeneration was confirmed by histology. Callusing was induced by culturing the mature embryos of the three rice varieties in a liquid medium of distilled water...
متن کاملIncreased Regeneration Efficiency in Seed Derived Callus of Rice (Oryza sativa L.)
In five cultivated rice varieties e.g., Nonabokra, PNR 381, Taipei 309, Koshika and TKM 11 the response in the initiation of callus and its size varied greatly among varieties, salt concentrations (NaCl and Na2SO4) and their interaction. Better regeneration ability of Nonabokra, Koshika and TKM 11 was found to be correlated to small size callus with a high concentration of Na2SO4. The regenerat...
متن کاملOptimizing tissue culture media for efficient transformation of different indica rice genotypes
An efficient system was established for high frequency embryogenesis and regeneration of indica rice, Oryza sativa L. cv. MDU 5. Basic media, carbohydrate sources and concentrations, agar concentrations, amino acids, cytokinins and auxins were evaluated in callus induction and regeneration media to establish the most efficient regeneration protocol for MDU 5 indica rice. The optimized callus in...
متن کاملAgrobacterium-mediated transformation of White Ponni, a non-basmati variety of indica rice (Oryza sativa L.)
We report successful Agrobacterium-mediated transformation of a popular rice variety White Ponni, a non-basmati indica rice (Oryza sativa L.). Scutellumderived calluses of White Ponni were transformed with Agrobacterium strain LBA4404 (pSB1) harbouring the binary vector pMKU-RF2 with rice chi11 gene. Five independent transgenic White Ponni plants were ge nerated from hygromycin-resistant callus...
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ژورنال
عنوان ژورنال: Physiology and Molecular Biology of Plants
سال: 2009
ISSN: 0971-5894,0974-0430
DOI: 10.1007/s12298-009-0042-6